GenCatch TM PCR Cleanup Kit


Catalog#sizePriceQuantity 
236005050 Preps $53.00
2360250250 Preps $215.00


Description

 PCR Cleanup Prep GenCatch TM PCR Cleanup Kit purifies DNA molecules from PCR and other enzymatic reactions from enzymes, dNTPs, salts, and primers without phenol/chloroform extraction. Recovery efficiency of DNA fragments ranging from 70 bp to 10 kb in size is up to 95%. It totally eliminate slurry carryover problem as encountered using silica beads or glass milk based methods. Up to 10 ¦Ìg DNA can be recovered with a single column and elution volumes can be as small as 10 ¦Ìl.

Detailed Components

 High capacity/quality columns with buffers and protocols. 

Features

Capacity: Up to 10 ¦Ìg of DNA.

Elution Volume: 10-50 ¦Ìl .

Recover Efficiency: Up to 95%.

Quality: DNA purified using GenCatch TM PCR Cleanup Kit is free from enzyme, polysaccharides, agraose, carry-over slurry and salt contamination. And is suitable for many downstream molecular manipulations including PCR, ligation, restriction enzyme digestion, transformation and transfection, radioactive and fluorescent sequencing.

Fast: Single samples can be prepared within 10  minutes with minimum efforts. 18-24 samples can be simultaneously processed in a centrifugation format within 30 minutes. Or, more can be processed using a vacuum manifold.

Flexible: Mini columns can used in either centrifugation procedure or using a vacuum manifold.

Convenient: No phenol extractions or ethanol precipitations required.

Technical Data

 PCR Cleanup Prep PCR products of various MW (lane 1-5)  were purified using GenCatch TM PCR Cleanup Kit and loaded as a cocktail (lane 6) onto 1.5% agraose gel. Recovery efficiency can be roughly estimated by comparing the overall intensity of the individual bands.