Description |
|
GenCatch TM Plant RNA Purification System provides a
simple,
fast and cost-effective method to purify RNA Plant and fugal samples. It
comes with shearing tubes for simple and fast homogenization and filtration of plant
tissues. It uses a simple silica-membrane spin-column method to isolate total RNA
greater than 200 nucleotides while excluding smaller RNA
such as 5S, 5.8S and tRNA
without phenol/chloroform extraction and ethanol precipitation. The system also
provides additional buffer for handling plant tissue that contai sticky secondary
metabolites. RNA isolated can be used for a variety of molecular manipulations such
as: Northern blotting, RT-PCR, PolyA+ RNA selection, cDNA synthesis,
Primer extension and in vitro translation, etc.
|
Detailed Components |
|
High quality spin columns, collection tubes together with buffers and protocols. |
|
Features |
|
|
Miniprep |
Maxiprep |
Sample Size |
up tp 100 mg plant tissue |
up to 1 g of plant tissue |
Binding Capacity |
up tp 100 ug |
up to 1 mg |
Elution Volume |
50 ul |
500 ul |
|
Technical Data |
|
Formaldehyde agarose gel analysis of total
RNA isolated from various plant samples with GenCatch TM Plant RNA Purification
System. Lane 1: Tobacco; 2: Arabidopsis; 3: Rice; 4: Tomato leaves; 5: Tomato stem;
6: Tomato root. 2 ug of total RNA was loaded per lane.
RT-PCR if total RNA extracted from different
tissue of tomato with GenCatch TM Plant RNA Purification System. Lane 1: total
RNA from leaves; 2: total RNA from stem; 3: total RNA from root; 1 kb DNA marker
is on the left.
|