Description |
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GenCatch TM Total RNA Extraction System provides an GenCatchmical method to purify
total RNA from various samples such as cultured cells, tissues, bacteria, and yeast.
A simple silica-membrane spin-column method can isolate total RNA without phenol/chloroform
extraction and ethanol precipitation. Total RNA with a molecular size greater than
200 nucleotides (which excludes smaller RNA such as 5S and 5.8S RNA, and tRNA) can
be isolated.
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Detailed Components |
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High quality spin columns, collection tubes together with buffers and protocols. |
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Features |
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Miniprep |
Midiprep |
Maxiprep |
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Sample Size
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Tissue
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10-30 mg |
100-200 mg |
0.5-1 g |
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Cell culture |
up to 1x 107 cells |
up to 3-7x 107 cells |
up to 2-10x 108 cells |
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Bacteria |
up to 1x 109 cells |
up to 1x 1010 cells |
up to 5x 1010 cells |
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Yeast |
up to 5x 107 cells |
up to 5x 108 cells |
up to 2x 109 cells |
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Yield
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Tissue |
up to 45 ug |
up to 450 ug |
up to 2.5 mg |
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Cell culture |
up to 30 ug |
up to 300 ug |
up to 6 mg |
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Bacteria/yeast |
up to 65 ug |
up to 650 ug |
up to 3 mg |
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Elution Volume |
200 ul |
500 ul |
2.5 ml |
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Technical Data |
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Total RNA isolated from tissue and cultured
cells using GenCatch TM Total RNA Extraction System was analyzed by formaldehyde
agarose gel electrophoresis.
RT-PCR of total RNA extracted from Hela cells
with GenCatch TM Total RNA Extraction System. Lane 1-5 are RT-PCR products
of the total RNA. Lane 6-10 are RT-PCR of total RNA extracted from 106,
105, 104, 103, and 102 HeLa cells respectively.
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