GenCatch TM Total RNA Extraction Kit

 

Catalog#sizePriceQuantity 
166005050 Mini Preps $153.00
1660250250 Mini Preps $702.00
167001010 Midi Preps $76.50
167005050 Midi Preps $333.00
16800066 Maxi Preps $99.00
168002424 Maxi Preps $342.00


Description

GenCatch TM Total RNA Extraction System provides an GenCatchmical method to purify total RNA from various samples such as cultured cells, tissues, bacteria, and yeast. A simple silica-membrane spin-column method can isolate total RNA without phenol/chloroform extraction and ethanol precipitation. Total RNA with a molecular size greater than 200 nucleotides (which excludes smaller RNA such as 5S and 5.8S RNA, and tRNA) can be isolated.

Detailed Components


High quality spin columns, collection tubes together with buffers and protocols. 

Features

Miniprep Midiprep Maxiprep
Sample Size      
Tissue 10-30 mg 100-200 mg 0.5-1 g
Cell culture up to 1x 107 cells up to 3-7x 107 cells up to 2-10x 108 cells
Bacteria up to 1x 109 cells up to 1x 1010 cells up to 5x 1010 cells
Yeast up to 5x 107 cells up to 5x 108 cells up to 2x 109 cells
Yield      
Tissue up to 45 ug up to 450 ug up to 2.5 mg
Cell culture up to 30 ug up to 300 ug up to 6 mg
Bacteria/yeast up to 65 ug up to 650 ug up to 3 mg
Elution Volume 200 ul 500 ul 2.5 ml

Technical Data


Total RNA Extraction

 

 

Total RNA isolated from tissue and cultured cells using GenCatch TM Total RNA Extraction System was analyzed by formaldehyde agarose gel electrophoresis.




Total RNA Extraction

RT-PCR of total RNA extracted from Hela cells with  GenCatch TM Total RNA Extraction System. Lane 1-5 are RT-PCR products of the total RNA. Lane 6-10 are RT-PCR of total RNA extracted from 106, 105, 104, 103, and 102 HeLa cells respectively.