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Protein Expression & Optimization
Epoch Life Science specializes in providing high-quality protein expression and purification services for pharmaceutical, genomics, and biotechnology companies. Our proteins are used for a variety of applications, including high throughput screening, crystallography, in vivo studies, and diagnostics. We offer a comprehensive range of services, from cloning to delivering purified protein, all with a focus on confidentiality, collaboration, and timely delivery.
We highly recommend taking advantage of our codon optimization service. Our advanced codon optimization algorithms are provided free of charge with your gene synthesis orders and ensure that your gene is optimized for maximum expression and functionality.
To demonstrate the benefits of codon optimization, we have included two case studies below that illustrate how it can enhance protein expression:
Figure Above: Codon optimization for E.coli expression.
Lane 1, Total cell extract of uninduced blank vector control; Lane 2, Total cell extract from un-optimized wild-type gene; Lane 3, Total cell extract from codon optimized gene; Lane 4, Supernatant of uninduced blank vector control; Lane 5, Supernatant from un-optimized gene; Lane 6, Supernatant from codon optimized gene. Target protein is ~60 kD.
Figure Below: Codon optimization for HEK293 cell expression.
10 randomly selected optimized sequences, together with the un-optimized wild-type gene were synthesized and expressed with His-tag in HEK293 cell. Protein expression level was measured using His-tag specific ELISA with serial diluted samples.
Our Protein Expression and Optimization services offer several advantages, including:
- Rapid Turnaround: We can deliver results in weeks, not months.
- Experience: We have produced hundreds of proteins from diverse classes.
- Multiple Expression Systems: We work with mammalian cells, insect cells, and E. coli.
- Capacity: We can produce milligram to gram quantities of purified protein.
- Quality: We provide batch records and certificates of analysis with every lot.
- Clear Communication: We offer detailed proposals with milestones.
- Customization: We format proteins for specific applications.
- Confidentiality: We strictly adhere to confidentiality requirements.
At Epoch Life Science, we are committed to providing excellent service that helps you increase productivity and conserve internal resources. Whether you require milligram or gram quantities of recombinant proteins, think of Epoch Life Science as your go-to choice.
For a quick project cost estimate, please contact us at: contractproteins@epochLifeScience.com
